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sulfo chromalink biotin  (Vector Laboratories)


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    Structured Review

    Vector Laboratories sulfo chromalink biotin
    Sulfo Chromalink Biotin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/B-1007/Sulfo+ChromaLINK+Biotin+(Water+Soluble)/bio_rxiv__2025__05__29__656795-347-25-28
    Average 96 stars, based on 4 article reviews
    sulfo chromalink biotin - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: IL-32 drives inflammatory responses in IFN-γ primed human macrophages via a Myddosome-dependent pathway and is elevated in COVID-19
    Article Snippet: .. Recombinant Human IL-32β [R&D Systems, 6769-IL] in 20 mM HEPES, 500 mM NaCl, 1 mM DTT, and 0.2% (w/v) CHAPS, pH 7.0, was labeled with Sulfo ChromaLINK® Biotin [Vector Lab, B-1007] at a 75X molar ratio for 90 minutes at room temperature to overcome reduced reactivity due to the presence of CHAPS and DTT. .. The sample was quenched with Tris, pH 7.0, and excess biotin was removed by dialysis using a Slide-A-Lyzer G2 Dialysis Cassette, 3.5K MWCO [Thermo Scientific, 87725], followed by one round of Zeba Spin Desalting Columns, 40K MWCO [Thermo Scientific, A57766].

    Labeling:

    Article Title: IL-32 drives inflammatory responses in IFN-γ primed human macrophages via a Myddosome-dependent pathway and is elevated in COVID-19
    Article Snippet: .. Recombinant Human IL-32β [R&D Systems, 6769-IL] in 20 mM HEPES, 500 mM NaCl, 1 mM DTT, and 0.2% (w/v) CHAPS, pH 7.0, was labeled with Sulfo ChromaLINK® Biotin [Vector Lab, B-1007] at a 75X molar ratio for 90 minutes at room temperature to overcome reduced reactivity due to the presence of CHAPS and DTT. .. The sample was quenched with Tris, pH 7.0, and excess biotin was removed by dialysis using a Slide-A-Lyzer G2 Dialysis Cassette, 3.5K MWCO [Thermo Scientific, 87725], followed by one round of Zeba Spin Desalting Columns, 40K MWCO [Thermo Scientific, A57766].

    Purification:

    Article Title: Targeted quantitation of CFTR protein expression in vivo using immunoprecipitation & parallel reaction monitoring tandem mass spectrometry
    Article Snippet: RapiGest SF surfactant was from Waters (Milford, MA). .. The purified anti-human CFTR antibody (shown as Table S2) was biotinylated using water-soluble Sulfo ChromaLink Biotin (Vector Laboratories, Burlingame, CA) via cross-linking biotin succinimidyl ester to antibody primary amine groups according to the manufacturer protocol. ..



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